Epidemiological investigation of Middle East respiratory syndrome coronavirus in dromedary camel farms linked with human infection in Abu Dhabi Emirate, United Arab Emirates
Identifieur interne : 001273 ( Main/Exploration ); précédent : 001272; suivant : 001274Epidemiological investigation of Middle East respiratory syndrome coronavirus in dromedary camel farms linked with human infection in Abu Dhabi Emirate, United Arab Emirates
Auteurs : Salama Al Muhairi [Émirats arabes unis] ; Farida Al Hosani [Émirats arabes unis] ; Yassir M. Eltahir [Émirats arabes unis] ; Mariam Al Mulla [Émirats arabes unis] ; Mohammed F. Yusof [Émirats arabes unis] ; Wissam S. Serhan [Émirats arabes unis] ; Farouq M. Hashem [Émirats arabes unis] ; Elsaeid A. Elsayed [Émirats arabes unis] ; Bahaaeldin A. Marzoug [Émirats arabes unis] ; Assem S. Abdelazim [Émirats arabes unis]Source :
- Virus Genes [ 0920-8569 ] ; 2016.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Cadres ouverts de lecture, Chameaux, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Fermes, Flambées de maladies, Humains, Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Ovis, Phylogénie, Émirats arabes unis (épidémiologie).
- MESH :
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- épidémiologie : Infections à coronavirus, Émirats arabes unis.
- Analyse de séquence d'ADN, Animaux, Cadres ouverts de lecture, Chameaux, Coronavirus du syndrome respiratoire du Moyen-Orient, Fermes, Flambées de maladies, Humains, Ovis, Phylogénie.
English descriptors
- KwdEn :
- Animals, Camelus, Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Disease Outbreaks, Farms, Humans, Middle East Respiratory Syndrome Coronavirus (classification), Middle East Respiratory Syndrome Coronavirus (genetics), Open Reading Frames, Phylogeny, Sequence Analysis, DNA, Sheep, United Arab Emirates (epidemiology).
- MESH :
- classification : Middle East Respiratory Syndrome Coronavirus.
- epidemiology : Coronavirus Infections, United Arab Emirates.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections.
- Animals, Camelus, Disease Outbreaks, Farms, Humans, Open Reading Frames, Phylogeny, Sequence Analysis, DNA, Sheep.
Abstract
The objective of this research was to investigate the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) infection primarily in dromedary camel farms and the relationship of those infections with infections in humans in the Emirate of Abu Dhabi. Nasal swabs from 1113 dromedary camels (39 farms) and 34 sheep (1 farm) and sputum samples from 2 MERS-CoV-infected camel farm owners and 1 MERS-CoV-infected sheep farm owner were collected. Samples from camels and humans underwent real-time reverse-transcription quantitative PCR screening to detect MERS-CoV. In addition, sequencing and phylogenetic analysis of partially characterized MERS-CoV genome fragments obtained from camels were performed. Among the 40 farms, 6 camel farms were positive for MERS-CoV; the virus was not detected in the single sheep farm. The maximum duration of viral shedding from infected camels was 2 weeks after the first positive test result as detected in nasal swabs and in rectal swabs obtained from infected calves. Three partial camel sequences characterized in this study (open reading frames 1a and 1ab, Spike1, Spike2, and ORF4b) together with the corresponding regions of previously reported MERS-CoV sequence obtained from one farm owner were clustering together within the larger MERS-CoV sequences cluster containing human and camel isolates reported for the Arabian Peninsula. Data provided further evidence of the zoonotic potential of MERS-CoV infection and strongly suggested that camels may have a role in the transmission of the virus to humans.
Url:
DOI: 10.1007/s11262-016-1367-1
PubMed: 27357298
PubMed Central: 7089110
Affiliations:
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Marzoug</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Veterinary services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates</nlm:aff><country xml:lang="fr">Émirats arabes unis</country><wicri:regionArea>Veterinary services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea><wicri:noRegion>Abu Dhabi</wicri:noRegion></affiliation></author><author><name sortKey="Abdelazim, Assem S" sort="Abdelazim, Assem S" uniqKey="Abdelazim A" first="Assem S." last="Abdelazim">Assem S. Abdelazim</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Veterinary services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates</nlm:aff><country xml:lang="fr">Émirats arabes unis</country><wicri:regionArea>Veterinary services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea><wicri:noRegion>Abu Dhabi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (virology)</term><term>Disease Outbreaks</term><term>Farms</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (classification)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Open Reading Frames</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Sheep</term><term>United Arab Emirates (epidemiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Chameaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Fermes</term><term>Flambées de maladies</term><term>Humains</term><term>Infections à coronavirus (virologie)</term><term>Infections à coronavirus (épidémiologie)</term><term>Ovis</term><term>Phylogénie</term><term>Émirats arabes unis (épidémiologie)</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>United Arab Emirates</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Émirats arabes unis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Disease Outbreaks</term><term>Farms</term><term>Humans</term><term>Open Reading Frames</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Sheep</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Chameaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Fermes</term><term>Flambées de maladies</term><term>Humains</term><term>Ovis</term><term>Phylogénie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The objective of this research was to investigate the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) infection primarily in dromedary camel farms and the relationship of those infections with infections in humans in the Emirate of Abu Dhabi. Nasal swabs from 1113 dromedary camels (39 farms) and 34 sheep (1 farm) and sputum samples from 2 MERS-CoV-infected camel farm owners and 1 MERS-CoV-infected sheep farm owner were collected. Samples from camels and humans underwent real-time reverse-transcription quantitative PCR screening to detect MERS-CoV. In addition, sequencing and phylogenetic analysis of partially characterized MERS-CoV genome fragments obtained from camels were performed. Among the 40 farms, 6 camel farms were positive for MERS-CoV; the virus was not detected in the single sheep farm. The maximum duration of viral shedding from infected camels was 2 weeks after the first positive test result as detected in nasal swabs and in rectal swabs obtained from infected calves. Three partial camel sequences characterized in this study (open reading frames 1a and 1ab, Spike1, Spike2, and ORF4b) together with the corresponding regions of previously reported MERS-CoV sequence obtained from one farm owner were clustering together within the larger MERS-CoV sequences cluster containing human and camel isolates reported for the Arabian Peninsula. Data provided further evidence of the zoonotic potential of MERS-CoV infection and strongly suggested that camels may have a role in the transmission of the virus to humans.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azhar, Ei" uniqKey="Azhar E">EI Azhar</name></author><author><name sortKey="El Kafrawy, Sa" uniqKey="El Kafrawy S">SA El-Kafrawy</name></author><author><name sortKey="Farraj, Sa" uniqKey="Farraj S">SA Farraj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nowotny, N" uniqKey="Nowotny N">N Nowotny</name></author><author><name sortKey="Kolodziejek, J" uniqKey="Kolodziejek J">J 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Muhairi</name></noRegion><name sortKey="Abdelazim, Assem S" sort="Abdelazim, Assem S" uniqKey="Abdelazim A" first="Assem S." last="Abdelazim">Assem S. Abdelazim</name><name sortKey="Elsayed, Elsaeid A" sort="Elsayed, Elsaeid A" uniqKey="Elsayed E" first="Elsaeid A." last="Elsayed">Elsaeid A. Elsayed</name><name sortKey="Eltahir, Yassir M" sort="Eltahir, Yassir M" uniqKey="Eltahir Y" first="Yassir M." last="Eltahir">Yassir M. Eltahir</name><name sortKey="Hashem, Farouq M" sort="Hashem, Farouq M" uniqKey="Hashem F" first="Farouq M." last="Hashem">Farouq M. Hashem</name><name sortKey="Hosani, Farida Al" sort="Hosani, Farida Al" uniqKey="Hosani F" first="Farida Al" last="Hosani">Farida Al Hosani</name><name sortKey="Marzoug, Bahaaeldin A" sort="Marzoug, Bahaaeldin A" uniqKey="Marzoug B" first="Bahaaeldin A." last="Marzoug">Bahaaeldin A. Marzoug</name><name sortKey="Mulla, Mariam Al" sort="Mulla, Mariam Al" uniqKey="Mulla M" first="Mariam Al" last="Mulla">Mariam Al Mulla</name><name sortKey="Serhan, Wissam S" sort="Serhan, Wissam S" uniqKey="Serhan W" first="Wissam S." last="Serhan">Wissam S. Serhan</name><name sortKey="Yusof, Mohammed F" sort="Yusof, Mohammed F" uniqKey="Yusof M" first="Mohammed F." last="Yusof">Mohammed F. Yusof</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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